John Johnstone
John Johnstone may refer to:
Politics
- John Johnstone (mayor) (1661–1732), mayor of New York, 1714–1719
 - Sir John Johnstone, 1st Baronet (died 1711), Scottish army officer and politician
 - John Johnstone (East India Company) (1734–1795), officer of the British East India Company, MP for Dysart Burghs 1774–80
 - Sir John Johnstone, 6th Baronet (1783–1811), British army officer and politician
 - John Heywood Johnstone (1850–1904), British barrister and Conservative Party politician
 - John Johnstone (Australian politician) (1857–1931), member of the Victorian Legislative Assembly
 - John Philemon Johnstone (1873–1960), member of the Ontario Legislative Assembly
 
Sports
- Jock Johnstone (fl. 1920s), Scottish footballer for Aston Villa
 - John Johnstone (athlete) (1892–1969), American Olympic track and field high jumper
 - John Johnstone (English footballer), English footballer for Port Vale
 - John Johnstone (footballer, born 1869) (1869–1953), Scottish footballer (Kilmarnock FC and Scotland)
 - John Johnstone (baseball) (born 1968), American professional baseball player
 
Other
- John Johnstone (physician) (1768–1836), British doctor and biographer
 - John Henry Johnstone (1749–1828), Irish actor and singer
 - John Johnstone (architect) (1818–1884), Scottish architect
 - John Johnstone (businessman) (1881–1935), British businessman and jockey
 - John Young Johnstone, Canadian Impressionist painter