The 2000 Tercera División play-offs to Segunda División B from Tercera División (Promotion play-offs) were the final playoffs for the promotion from 1999–2000 Tercera División to 2000–01 Segunda División B. The first four teams in each group (excluding reserve teams) took part in the play-off.
The 68 participating teams were divided into 5 series each made up of 4 groups in the category, with the exception of  Series E , which was only formed by  Group XII . Each series was divided into 4 groups formed by a 1st, a 2nd, a 3rd and a 4th classified from each group, which played a double-round playoff. Each victory was equivalent to 3 points, the tie to 1 point and the defeat to 0 points. The champion of each group obtained the promotion to Second Division B.
The distribution of each series was as follows:
Teams for 1999–2000 play-offs
Tables and Results
Group A-1
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group A-2
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group A-3
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group A-4
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group B-1
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group B-2
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group B-3
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group B-4
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group C-1
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group C-2
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group C-3
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group C-4
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group D-1
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group D-2
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group D-3
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group D-4
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
Group E
Updated to match(es) played on completed. Source: 
FutbolMeLegend: Blue = home team win; Yellow = draw; Red = away team win.
  
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 Group I – Galicia   
 
Group II – Asturias   
 
Group III – Cantabria   
 
Group IV – Basque Country   
 
Group V – Catalonia   
 
Group VI – Valencian Community  
 
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 Group VII – Community of Madrid   
 
Group VIII – Castile and León   
 
Group IX – E. Andalusia and Melilla    
 
Group X – W. Andalusia and Ceuta    
 
Group XI – Balearic Islands   
 
Group XII – Canary Islands   
 
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 Group XIII – Region of Murcia   
 
Group XIV – Extremadura   
 
Group XV – Navarre and La Rioja    
 
Group XVI – Aragon   
 
Group XVII – Castilla–La Mancha   
 
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